Results and Discussion Example

Results In this essay we utilize paper chromato chartical recordy to determine the amount of chlorophyl a, chlorophyll b, xanthophyll, and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table denominate table 1. display board 1 introduces the transmission at individually wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used with the help of dimethyl ketone we saw the spectrum and the differences of the contrastive pigments.Percentage transmittance in Table 1 show that the dispiriteder the number, the more dense that solution is at that specific wavelength. As the numbers get higher, the less dense it is and if the number is century at a specific wavelength then the solution was clear. As we can uplift from Table 1, severally of the pigments had different low transmittance based on how high the absorption was in each of the pigments. For this we calculated that at at that place was a backsheesh on the graph at the lowest points in table 1.For instance, in the chlorophyll a column in Table 1 the lowest points on the graphs at 8 and 28 and as well at 44, which shows that on that point give be a item rough those points. In chlorophyll b, the lowest points are from 25. 2, 12. 4, and 9. 4, which would be the first peak and the next will be at 55. 6. In Xanthophyll, the points were the first peak will be are 52. 4, 43. 6, 44. 8, and 53. 0. Lastly in Carotene, the graph will peak at 92. 2, 93. 6, and 92. 4. By using these points we predicted the behavior of the graph.TABLE 1 Wavelength chlorophyl a chlorophyll b Xanthophyll Carotene 400 12 42 61. 8 96. 8 420 8 25. 2 52. 4 99. 6 440 28 12. 4 43. 6 92. 2 460 71. 5 9. 4 44. 8 93. 6 480 81 48. 4 53 92. 4 euchre 88 88. 6 83. 2 99. 2 520 88 91. 4 94. 2 ampere-second 540 84. 5 87. 4 95. 6 100 560 83 85. 2 96. 4 99. 2 580 71. 5 83. 4 96. 2 100 600 72. 5 76. 8 96 100 620 61. 5 78. 2 94. 4 100 640 61. 5 59. 6 94. 6 100 660 44 55. 6 93. 2 100 680 48. 8 67. 8 92. 6 99. 8 700 64. 6 60 91. 4 92. 8 720 50. 6 52. 4 89 -On the graph denominate Figure 1, it shows the seize onance of each of the pigments that are sh consume in Table 1. The difference mingled with Table 1 and Figure 1 is that where table 1 shows the transmittance points of each wavelength of each pigment, Figure 1 shows the absorption peaks of each of the wavelengths. For chlorophyll a, the highest peaks are at 420, but then the graph plummets at or so 450 and has some other peak between 650 and 700. For chlorophyll b, the highest peaks of absorbance are between 450 and 500 then it actualizes a big plummet around 500 and has another peak between 640 and 660.For Xanthophyll, the only peak is between 420 and 480, then the graph as minimal movement from there and stays in the 90 set forth never hitting 100. Lastly, beta-carotene has small peaks at 440 and 460 and then the graph stays almost stagnant at 100. Di scussion The purpose of this experiment was to find out why the leaves on a corner changed blazon in the fall. We predicted that the molecules in the leaves of the tree attributed to the change. We found out that the molecules Chlorophyll a, Chlorophyll b, Xanthophyll, and Beta-carotene are the main molecules that reside in a leaf.We also verbalise that the tree absorbs the elements found in some of these molecules so that they can be used in the spring, when the trees are green once more. This prediction came from when we canvas the molecular spell of each of the molecules and correlated the results the experiment and the specific elements that the tree would have needed for another year. The results of this experiment show that at there is less chlorophyll a and chlorophyll b in the composition of the leaves that were used.This meant that when the chasten is changing, the molecules in these structures start to be absorbed back into the tree. This certainty came from the fac t that in table 1 from 420 and 440 Nano meters there was a low transmittance rate of these two molecules, which means that the absorbance rate was high. During the fall season there is not enough sunlight for the trees to perform photosynthesis to make their own food, what a plant needs to survive would be the Magnesium and nitrogen that are found in the molecular structures of chlorophyll a and b.Therefore they absorb all of these nutrients that the tree needs to survive so that they can make it thorough the winter. Errors could have been made with this experiment. Some of them include that the individuals in different groups did not have the same control as the others. We did not broadsheet the same amount of acetone in each vial nor did we treasure how much of the leaf extraction each person used. Another rootage of error would be how the spectrometer was used, if it was reset the same at each wavelength each time.Compared to previous experiments the information that was coll ected in this one was accurate. The peaks for each pigment were roughly around the same wavelength. The standard for chlorophyll a was peaks at 430 and 662, chlorophyll b peaks at 453 and 642, xanthophyll a range from 450 to 550 and for the beta-carotene peaks at a range from 450 to 550. Our results were roughly around the same as you can see from Table 1. The end result of the experiment was successful, because of the results that we produced were homogeneous to the results of previous experiments.